Table 3.
Open reading frames (ORF) present in invasive strains but absent at least in two out of three commensal tester group B Streptococcus strains by comparative genomic hybridization and their presence among 949 GBS isolates and their prevalence ratio between invasive (n = 386) and colonizing (n = 563) isolates.
| ORF | Probe-positive strains (%) | Prevalence ratio (95% CI)(a) | Predicted protein |
|---|---|---|---|
| sag0004 | 524 (55%) | 1.1 (0.96–1.22) | hypothetical protein |
| sag0005 | 706 (74%) | 1.0 (0.93–1.09) | hypothetical protein |
| sag0027 | 941 (99%) | 1.0 (0.98–1.00) | phosphoribosylaminoimidazole synthetase |
| sag0175 | 692 (73%) | 1.0 (0.93–1.09) | hypothetical protein |
| sag0206 | 590 (62%) | 0.9 (0.82–1.01) | lipoprotein, putative |
| sag0253 | (b) | (b) | acetyltransferase, GNAT family |
| sag0414 | 927 (98%) | 1.0 (0.97–1.01) | phosphorylase, Pnp/Udp family, putative |
| sag0426 | — | — | cupin family protein |
| sag0427 | 517 (54%) | 1.0 (0.93–1.17) | transcriptional regulator, MerR family |
| sag0700 | 925 (97%) | 1.0 (0.97–1.02) | 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase |
| sag0814 | 117 (12%) | 0.6 (0.39–0.83) | hypothetical protein |
| sag0815 | 364 (38%) | 0.9 (0.76–1.06) | transcriptional regulator, Cro/CI family-related protein |
| sag0832 | 371 (39%) | 1.5 (1.29–1.77) | fibrinogen binding protein |
| sag1130 | 367 (39%) | 1.1 (0.90–1.25) | hypothetical protein |
| sag1140 | (b) | (b) | hypothetical protein |
| sag1207 | (b) | (b) | hypothetical protein |
| sag1781 | (b) | (b) | primase-related protein |
| sag1968 | 87 (9%) | 1.1 (0.71–1.61) | hypothetical protein |
| sag1969 | 907 (96%) | 1.0 (0.97–1.03) | ribosomal protein L11 methyltransferase |
| sag1974 | (b) | (b) | MutT/nudix family protein |
| sag1975 | (b) | (b) | hypothetical protein |
| sag1976 | 290 (31%) | 0.9 (0.73–1.08) | hypothetical protein |
| sag1994 | 289 (30%) | 1.0 (0.82–1.22) | hypothetical protein |
| sag1999 | (b) | (b) | hypothetical protein |
| sag2021 | 395 (42%) | 1.2 (1.00–1.36) | cell wall surface anchor family protein |
| sag2026 | 224 (24%) | 1.1 (0.88–1.40) | membrane protein, putative |
| sag2027 | (b) | (b) | ABC transporter, ATP-binding protein |
| sag2028 | (b) | (b) | hypothetical protein |
| sag2045 | 364 (38%) | 1.1 (0.90–1.25) | DNA topology modulation protein FlaR, putative |
| sag2057 | (b) | (b) | leucyl-tRNA synthetase |
| sag2060 | 427 (45%) | 1.3 (1.13–1.50) | glycosyl transferase, family 8 |
| sag2061 | 437 (46%) | 1.2 (1.07–1.41) | glycosyl transferase, family 8 |
| sag2088 | (b) | (b) | hypothetical protein |
| sag2147 | 687 (72%) | 1.0 (0.9–1.06) | lipoprotein, putative |
| gbs0474(c) | 270 (28%) | 1.5 (1.21–1.80) | hypothetical protein |
(a)Prevalence with confidence interval not overlapping 1 are considered statistical significant.
(b)These ORFs were not screened using LOS mostly because of small sizes.
(c)Exact corresponding gene in strain 2603VR was not found by blast search while it was classified as present in 2603 VR by CGH.