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. 2009 Feb 23;119(3):551–564. doi: 10.1172/JCI36604

Figure 2. Degree of difference in IL-2 production and proliferation between Tregs and Teffs in the site of infection.

Figure 2

(A) Representative flow cytometry analysis of HCV-PBLs or HCV-IHLs stained with mAbs to CD4, CD25, and Foxp3, stimulated or not with anti-CD3/CD28, and processed to detect intracellular IL-2. Contour plot analyses are gated on CD4+CD25+ cells and show percentages of double-stained IL-2+Foxp3+ cells. The percentage of cells is indicated in each quadrant. (B) Percentage of IL-2+ cells (analyzed by flow cytometry as in A) in peripheral or intrahepatic cell populations. Values subtracted the background are shown. (C) Representative flow cytometry analysis of HCV-PBLs or HCV-IHLs stained with mAbs to CD4, CD25, Foxp3, and Ki67. Contour plot analyses are gated on CD4+CD25+ cells and show cells stained with mAbs to Ki67 and Foxp3. The percentage of cells is indicated in each quadrant. (D) Percentage of Ki67+ cells (analyzed by flow cytometry as in C) in peripheral or intrahepatic cell populations. In B and D, statistical analyses of values between HCV-IHLs and HCV-PBLs were performed with the nonparametric Mann-Whitney U test for paired data, whereas those between cell populations from HCV patients and HDs were performed with the nonparametric Mann-Whitney U test for unpaired data. *P < 0.05; **P < 0.0065; ***P < 0.0015. Each symbol represents a single individual.