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. 2009 Feb 9;119(3):512–523. doi: 10.1172/JCI35070

Figure 5. Validation of acute and chronic lung FGF2 knockdown by siRNA.

Figure 5

(A) FGF2 mRNA and protein levels in rat lung 12 hours and 120 hours after MCT injection. Rats were injected with FGF2-siRNA or the scrambled siRNA sequence either once, 3 days before MCT injection (12-hour group), or twice, 3 days before and 1 day after MCT injection (120-hour group). (B) FGF2 mRNA and protein levels in rat lungs 21 days after MCT injection. Rats were injected with FGF2-siRNA or the scrambled siRNA sequence (2 [2iv] or 4 [4iv] injections). FGF2 mRNA levels were assessed using real-time quantitative PCR and protein levels with FGF2 immunoblotting (the lanes were run on the same gel but were not contiguous). All values are mean ± SEM from at least 5 animals in each group. *P < 0.05; **P < 0.01; ***P < 0.001 vs. rats injected with saline instead of MCT or vs. rats treated with the scrambled siRNA sequence.