Figure 1. High-fat diet increases the mitochondrial H₂O₂ emission potential in skeletal muscle.

(A) Representative trace comparing rates of mitochondrial H₂O₂ emission under state 4 conditions (10 μg/ml oligomycin) in permeabilized skeletal muscle fibers prepared from a red gastrocnemius muscle of rats fed standard chow (Std chow), high-fat diet (HF; lard) for 3 days, or a high-fat (60%) diet for 3 weeks. The experiment was initiated by addition of glutamate/malate (GM, 5 μM/2 μM) to a deenergized fiber bundle (FB), followed by successive additions of succinate (final concentration shown in mM). (B) Quantified rates of experiments shown in A. Data represent mean ± SEM; n = 4, *P < 0.05 vs. Std chow.