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. 2009 Feb;15(2):346–354. doi: 10.1261/rna.1257509

FIGURE 6.

FIGURE 6.

Fluorescence spectra of PRE (A), POST (B), and POST2 (C) complexes. DA, Phe-tRNAPhe(Cy3)+fMet-tRNAfMet(Cy5); DU, Phe-tRNAPhe(Cy3)+fMet-tRNAfMet; and UA, Phe-tRNAPhe+fMet-tRNAfMet(Cy5). (A) PRE complexes (0.1 μM in Buffer C); (B) POST complexes formed by mixing PRE complexes with 0.5 μM EF–G and 1 mM GTP; (C) POST2 complexes formed by mixing PRE complexes with 0.5 μM EF-G, 1 mM GTP, 0.3 μM Lys-tRNALys, and 0.5 μM EF-Tu. All mixtures were incubated at 25°C for at least 3 min prior to recording spectra. Each spectrum is an average of three traces. All concentrations are final.