Skip to main content
. 2009 Feb;15(2):215–223. doi: 10.1261/rna.1374809

FIGURE 2.

FIGURE 2.

Construction of the ΔrrsA∷ htk1 knockout strain. (A) The 16S rRNA gene rrsA is represented by the black box, with the surrounding chromosomal regions in white. The htk gene is represented by the gray box. Arrows indicate the direction of transcription. Dashed lines indicate the limits of sequence identity between the two chromosomal loci. Arrowheads indicate the positions of primers used for diagnostic PCR analysis; A, primer Tth P16S-1; B, primer Tth T16S-3; C, primer HTK-1; D, primer HTK-2. (B) Diagnostic PCR analysis of the chromosomal rrsA locus of HB8 and HG 286. L, size markers; wt, DNA from wild-type HB8; Δ, DNA from the ΔrrsA∷ htk1 mutant HG 286. Predicted sizes of the PCR products in base pairs are indicated below the gel.