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Structure probing of wild-type and mutant ribosomes in the helix 27 region of 16S rRNA with DMS. Wild-type ribosomes (lane 1); U13C (lane 2); U20G (lane 3); C912A (lane 4); A914G (lane 5); and A915G (lane 6). K, unmodified ribosomes; DMS, modification with dimethylsulfate; DSM–N7, modification with dimethylsulfate followed by reduction with NaBH4 and cleavage with aniline to detect modification at the N7 of Gs. Arrowheads indicate the positions of C912, A914, and A915.
