FIGURE 2.
HPLC-UV chromatograms of the four samples. The guanosine signal has the same height in all chromatograms, wherefore the relative intensities of other signals are directly comparable. Peaks marked with an asterisk are sample preparation artifacts present in an enzyme-only hydrolysis, and the peaks marked with $ are unknown contaminants of nonnucleoside nature. The peaks corresponding to the expected modified adenosines are marked for visualization purposes.