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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: J Infect Dis. 2008 Sep 15;198(6):836–842. doi: 10.1086/591186

Figure 1.

Figure 1

Western blot of presumptive hMPV negative and new infection sera. Purified hMPV sFA1, sFA2, and sFB1 and RSV sF were resolved on 4-12% Nu-PAGE gels and transferred to nitrocellulose membranes. The membranes were probed with human sera as follows: (A) acute and convalescent sera from a patient after the acute sample tested negative but the convalescent sample tested positive for hMPV sF antibody in ELISA, or (B) RSV positive convalescent sera from a patient that was ELISA negative for hMPV sF in both acute and convalescent sera. Bound antibodies were reacted with a peroxidase conjugated goat anti-human IgG and visualized by chemiluminescence.