Table 2.
BMT | Marrow Prep and Pre-condition | 1 Month Post-HSCT | 2 Months Post-HSCT | At least 3 Months Post-HSCT | ||||
---|---|---|---|---|---|---|---|---|
T cells, %* | B cells, %* | T cells, %* | B cells, %* | T cells, %* | B cells, %* | Granulocytes, %† | ||
Congenic (CD45.1) | HSC‡ (n=6) | 4.0±2.7 | 0 | 16.3±4.1 | 0 | 64.4±8.2 | 0 | N/A |
Congenic (CD45.1) | HSC‡/TBI (n=4) | 44.6±3.5 | 18.4±2.8 | 56.6±4.5 | 23.5±7.0 | 46.1±2.2 | 35.2±2.1 | 64.1±11.2 |
Allogeneic (BALB/c) | HSC‡ (n=5) | 0 | 0 | 0 | 0 | not available | not available | not available |
Allogeneic (BALB/c) | HSC‡/TBI (n=4) | 1.8±1.2(3)£ | 3.3±1.5(3)£ | 4.4±3.4(2)£ | 3.4±0.7(2)£ | not available | not available | not available |
WT mice used as controls had a mean percent of 28.5±17.7 CD3+ (T cells) and a mean percent of 37.5 ± 2.1 CD45R/B220+ (B cells) in the CD45+ lymphocyte gate.
Mean percent of CD3+ (T cells) or CD45R/B220+ (B cells) in CD45+ lymphocyte gate ± standard deviation.
Mean percent of donor granulocytes of total granulocytes ± standard deviation.
For HSC (lin−c-kit+), 1 × 105 cells were injected. Mice treated with TBI (Total Body Irradiation) received 3Gy.
Mean±SD of engrafted animals. ( ) = the number of transplanted mice that engrafted (≥0.75% donor cells).