Figure 6.

Overexpression of jet in clock neurons suppresses the defects in phase shifts to light of flies with dominant-negative CSN5. Phase shifts were measured and plotted as in Figure 5. Either Pdf_0.5-Gal4, with two copies of the driver, or tim-Gal4 was used to express UAS-CSN5^DN4 alone, UAS-jet alone, or both transgenes together. The data for Pdf > +, Pdf > CSN5^DN4 and tim > CSN5^DN4 are replotted from Figure 5. For the new genotypes, each bar represents at least 15 flies analyzed in at least two independent experiments. Overexpression of jet via Pdf_0.5-Gal4 or tim-Gal4 has no effect on phase shifts compared with controls (p > 0.15). Phase shifts are reduced in Pdf > CSN5^DN4 flies compared with Pdf > CSN5^DN4 + jet flies (p < 0.0001) and between tim > CSN5^DN4 flies and tim > CSN5^DN4 + jet flies (p < 0.003).