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. Author manuscript; available in PMC: 2009 Feb 27.
Published in final edited form as: J Biol Chem. 2006 Oct 12;281(50):38543–38554. doi: 10.1074/jbc.M605734200

FIGURE 8. Western blot (IB) analysis of heterodimerization of α2B-AR and M6A or Y12A/S13A mutants.

FIGURE 8

A, dimerization of α2B-ARand M6A. HEK293T cells were transfected with HA-tagged α2B-AR plus the pEGFP-N1 vector (control), GFP-tagged α2B-AR (WT) or GFP-tagged M6A. IP, immunoprecipitation. B, dimerization of α2B-AR and Y12A/S13A. HEK293T cells were transfected with HA-α2B-AR together with the pEGFP-N1 vector (control), GFP-tagged α2B-AR (WT) or GFP-tagged Y12A/S13A. The cells were solubilized and immu-noprecipitated with anti-GFP antibodies. The immunoprecipitate was sepa-rated by SDS-PAGE, and immunoprecipitated receptors were revealed by Western blotting with anti-HAantibodies (upper panels).The blots were then stripedand reprobed with anti-GFP antibodies (lower panels).The data shown in A and B are representative of three independent experiments.