Figure 8.

A) In vivo incorporation of ⁵⁵Fe into [4Fe-4S] cluster-containing FNR-L28H (circles) and clusterless FNR-L28H-C122S (triangles). pACYC184-derived FNR-L28H and FNR-L28H-C122S were immunoprecipitated from 1 ml aliquots of aerobically (open symbols) or anaerobically (closed symbols) grown cultures. The total DPM per ml for ⁵⁵Fe was calculated as described in the Methods. The error bars represent the standard errors of triplicate samples. B) Growth measured by the optical density at 600 nm, of the aerobically (open circles) or anaerobically (closed circles) ⁵⁵Fe-labelled cultures expressing FNR-L28H.