Fig. 6.

Dominant negative and constitutively active forms of Rab11 localize to distinct membrane compartments. Vero cells were transfected with cDNAs expressing eGFP-Rab11 (A) wild-type, (B) S25N, or (C) Q70L proteins (green). At 18 hpt, cells were fixed and immunostained for the trans-Golgi marker, TGN46 (red, AlexaFluor 594). Nuclei were counterstained with TO-PRO-3 (blue) and are shown in the merged images only. All images are flattened reconstructions of confocal z-stacks acquired at 63× optical magnification and 2× digital zoom.