Table 1.

Inhibition of clonal proliferation of tumor cell lines by vitamin D₃ analogs

Cell lines	1,25(OH)2D3	Deuterated Gemini Vitamin D3 (BXL-01-)
		0098	0114	0101	0117	0108	0120
HL-60	7.4 × 10−11	1.0 × 10−12	9.1 × 10−12	1.6 × 10−13	1.4 × 10−13	5.1 × 10−13	1.4 × 10−13
LNCaP	1.9 × 10−11	6.4 × 10−11	2.5 × 10−10	4.9 × 10−13	3.2 × 10−13	5.8 × 10−11	1.5 × 10−12
PC-3	8.0 × 10−10	1.1 × 10−10	1.4 × 10−10	8.8 × 10−13	<1.0 × 10−13	3.4 × 10−12	4.1 × 10−13
DU145	N. R.	1.8 × 10−8	3.8 × 10−9	2.9 × 10−9	N.R.	1.5 × 10−8	4.6 × 10−8
H520	2.1 × 10−9	1.3 × 10−10	1.8 × 10−10	9.8 × 10−12	1.4 × 10−12	1.8 × 10−10	7.4 × 10−12
HT-29	5.8 × 10−10	2.8 × 10−10	1.1 × 10−10	1.9 × 10−11	3.8 × 10−12	1.3 × 10−10	9.4 × 10−11
MCF-7	3.1 × 10−9	4.9 × 10−9	2.1 × 10−10	1.0 × 10−11	2.4 × 10−12	1.2 × 10−10	4.0 × 10−11

Dose-response clonogenic assays in soft agar were performed; the data were plotted on semilogarithm graphs, and the curves were used to calculate the concentration of the analogs achieving a 50% inhibition (ED₅₀) of clonal growth. N.R.: ED₅₀ was not reached at 10⁻⁷ M analog. The analogs are abbereviated by their last digits which are shown on Fig. 1.