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. Author manuscript; available in PMC: 2009 Sep 9.
Published in final edited form as: Biochemistry. 2008 Aug 15;47(36):9355–9364. doi: 10.1021/bi800725q

Figure 5.

Figure 5

Ku restores the helicase activity of UvrD2-(1-589). Helicase reaction mixtures (10 μL) containing 5 mM MgCl2, 50 nM 3′-tailed duplex DNA substrate, 1 mM ATP, either 100 ng of UvrD2-(1-589) or 100 ng of UvrD1 (where indicated by +), and Ku as specified were incubated for 5 min at 37 °C. The products were analyzed by native PAGE and visualized by autoradiography. A control reaction without added protein is included in lane -E; a control reaction lacking enzyme that was heat denatured prior to PAGE is shown in lane Δ. The fraction of the input radiolabeled DNA corresponding to the unwound single strand was quantified by scanning the gel; the values are shown below each lane.