Table 10.1.

Representative oligonucleotide sequences utilized for the poly d(T) and TC primers for the TC RNA amplification method

Antisense RNA orientation

First-strand synthesis primer (66 bp): 3′-AAA CGA CGG CCA GTG AAT TGT AAT ACG ACT CAC TAT AGG CGC TTT TTT TTT TTT TTT TTT TTT TTT -5′

TC primer (17 bp): 5′-TAT CAA CGC AGA GTC CC -3′

Sense RNA orientation

First-strand synthesis primer (18 bp): 3′-TTT TTT TTT TTT TTT TTT -5′

TC-T7 primer (51 bp): 5′-AAA CGA CGG CCA GTG AAT TGT AAT ACG ACT CAC TAT AGG CGC GAG AGC CCC -3′