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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Gastroenterology. 2008 May 15;135(2):610–620. doi: 10.1053/j.gastro.2008.05.032

Figure 2.

Figure 2

Expression of contractile and related regulatory proteins in jejunum from MLCKSMKO mice. Western blot of ILK, ROCK1, MYPT1, RLC, SM-MHC and telokin (A and C) from MLCKSMKO (KO) and CTR jejunum. The samples were resolved by separate SDS-PAGE and total actin stained with Coomassie brilliant blue was used as loading control. MYPT1 phosphorylations at residues 696 and 850 in response to ACh were measured by anti-MYPT1-P696 and anti-MYPT1-P850 antibody (B). Ileal total proteins (30 μg) in MLCKSMKO and CTR were resolved by SDS-PAGE and stained with Coomassie brilliant blue (left side of D). To visualize more clearly the total myosin heavy chain and actin, we performed electrophoresis with different amounts of protein loading (100 μg) for comparisons of KO and CTR samples (right side of D).