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. Author manuscript; available in PMC: 2010 Jan 9.
Published in final edited form as: Brain Res. 2008 Oct 21;1247:62–70. doi: 10.1016/j.brainres.2008.10.008

Figure 1.

Figure 1

Placements for all successful brain microinjections. A, B: Raphe magnus (RM) injections for experiments shown in Fig. 2 (A) and Figs. 4–5 (B) are depicted in drawings of three coronal sections (−10.0, −10.8 and −11.3 mm from bregma, labeled far left in A). Actual AP placements ranged from −9.68 to −10.04, −10.3 to −11.0, and −11.3 to −11.6, respectively. C, D: Periaqueductal grey (PAG) injection sites for experiments shown in Fig. 3 (C) and Figs. 4–5 (D) are depicted in drawings of −7.8, −8.0 and −8.3 mm from bregma (labeled far right in D). Actual AP placements ranged from −7.3 to −7.8 (for −7.8 plate) and −8.3 to −9.16 (for −8.3 plate). Placements shown in the left PAG were from improgan-treated subjects displaying mild motor changes (EMB, n=5 in C, n=2 in D, see Results); those drawn in the right PAG showed no such effects. Symbols represent each subject’s experimental group, designated here as in the legends of subsequent figures. Open circle: Veh/Veh; open triangle: Veh/Imp; filled circle: SR/Veh; filled triangle: SR/Imp [Figs. 2, 4, same as SR20/Imp in Fig. 3]; open square: Veh/Win; filled square: SR/Win; asterisk: SR5/Imp; filled diamond: SR40/Imp. Section drawings are from (Paxinos and Watson, 1986).