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. Author manuscript; available in PMC: 2009 Dec 31.
Published in final edited form as: J Am Chem Soc. 2008 Dec 31;130(52):17867–17873. doi: 10.1021/ja8059057

Figure 5.

Figure 5

Effect of binding of M13 peptide with Ca2+-CaM-ELP60 on LCST behavior of the ELP fusion protein. a) Turbidity at 350 nm (OD350) as a function of solution temperature for Ca2+-CaM-ELP60 (filled squares, blue), Complex of Ca2+-CaM-ELP60 with M13 peptide, M13 (filled circles, green). b) Control of self-assembly of CaM-ELP fusion upon M13 peptide binding. The OD350 was measured as a function of time after sequential injection of Ca2+ followed by M13 peptide in a ratio of fusion protein:peptide (1:1) as indicated by the arrows.