Table III.
Immunohistochemical staining of γ-H2AX and analysis of apoptosis in cells exposed to hypotonic salt
| Hypotonic incubation (min) | Recovery time (min) | Nuclear H2AX staininga,b |
Fold change in annexinV (+) cellsc,d | ||
| % Strong | % Medium | % Weak | |||
| 0 | 30–120 | 0 | 0 | 4.2 ± 1.1 | 0.00 |
| 30 | 30 | 8.8 ± 1.6 | 14.7 ± 2.0 | 29.6 ± 3.1 | 0.66 ± 0.12 |
| 120 | 0.86 ± 0.06 | ||||
| 60 | 30 | 7.5 ± 0.8 | 12.1 ± 3.3 | 29.01 ± 5.2 | 0.87 ± 0.24 |
| 120 | 1.0 ± 0.40 | ||||
| 90 | 30 | 7.6 ± 1.4 | 17.2 ± 2.1 | 40.8 ± 5.6 | n/d |
| 120 | 30 | 3.75 ± 2.0 | 12.3 ± 2.6 | 48.1 ± 7.4 | n/d |
n/d, not deteremined.
a
≥1000 total cells were analysed after each treatment.
b
Total background staining was 4.2% (weak) from 0.5 to 2 h in isotonic medium.
c
Data averaged from cerebellar and subventricular neural precursor cells.
d
Fold change in the number of annexin V-positive cells compared to controls.