Figure 10.

β-arrestin 2^{K18R, K107R, K108R, K207R, K296R}demonstrates no co-localization with M₁ mAChR in MEF KO1/2. MEF KO1/2 cells were transfected with eGFP-M₁ (A) mAChRs (green) and either wild-type FLAG-β-arrestin 2, FLAG-β-arrestin 2^{K18R, K107R, K108R, K207R, K296R} or FLAG-β-arrestin 2^{K11R, K12R} (red). 24 hr after transfection, cells were treated with 1 mM carbachol for 30 min. Cells were fixed, probed, and imaged as described in methods. None of the β-arrestin constructs demonstrate co-localization with the M₁ mAChR. Image shown is representative of two independent experiments.