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(a) WNV protease demonstrated substrate inhibition of the small peptide substrate Boc-Gly-Arg-Arg-AMC. (b) WNV protease demonstrated substrate inhibition of the longer peptide substrate Ac-Asp-Phe-Ala-Ser-Gly-Lys-Arg-AMC. Data was analyzed with the program Dynafit according to Scheme 1. Curves representing the substrate inhibition and the classical Michaelis-Menten models are indicated by solid and dashed lines, respectively. Initial velocity data points showed < 2% error.
