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(a) The peptide Ac-GRR-NH2 inhibits WNV NS2B-NS3 protease according to a substrate inhibition and mixed inhibition model. Concentrations of inhibitor tested were 0 (triangles), 350 (circles), 700 (squares) μM, and 1 mM (diamonds). Data was analyzed with the program Dynafit according to Scheme 2. (b) Curves reflect an analysis of Ac-GRR-NH2 inhibition utilizing a mixed inhibition model without substrate inhibition. Initial velocity data points showed < 2% error.
