Figure 4. Double mutant petite frequency phenotypes.

Based on their persistence as strongly predicted candidates during our second iteration, we selected 26 genes unconfirmed by single mutant analysis for investigation of synthetic phenotypes. The single mutant petite frequency is shown for each of these strains on the left. Each of the 26 strains was crossed with 4 genes known to be involved in mitochondrial transmission (aim17Δ, tom6Δ, rvs167Δ, and ehd3Δ) to create ∼100 double mutant strains. Results are shown in blue for each of the 4 strains crossed into, followed by all 26 double mutants constructed against that strain (raw data Table S7). The order of the double mutants is the same as in the 26 single mutants shown on the left. Colors are as in Figure 3. Significantly altered double mutant strains are marked with numbers, corresponding to the key above the box plots.