Skip to main content
. 2009 Mar 6;4(3):e4722. doi: 10.1371/journal.pone.0004722

Figure 4. The RNA in ESMVs is not degraded.

Figure 4

Real time quantitative RT-PCR was used to measure the level of degradation of oct-4 and GFP mRNAs in ESMVs by comparing the 5′ and 3′ amplicon ratios of these transcripts in ESMVs with those in ESCs. Significant levels of degradation were not detected with either transcript. (A) Box plot of normalized 5′ and 3′ template values for oct-4 mRNA in ESCs and ESMVs (n = 9). (B) Box plot of normalized 5′ and 3′ template values for GFP mRNA in ESCs and ESMVs (n = 12). The boxed area represents the mean±quartile and the whiskers extend out to the minimum and maximum values. Bootstrap t-tests were performed to compare the 5′:3′ ratios for each transcript in ESCs and ESMVs. No significant difference was detected between the ESC group and ESMV group for either transcript (p>0.1).