Table 1. Primers used for RT-PCR.
Primer pairs | Sequence (5′–3′) | Amplicon (bp) |
Oct-4 5′ | GCCGGGCTGGGTGGATTCTC | 271 |
ATTGGGGCGGTCGGCACAGG | ||
Oct-4 3′ | AGGCCCGGAAGAGAAAGCGAACTA | 265 |
TGGGGGCAGAGGAAAGGATACAGC | ||
GFP 5′ | ATGGTGAGCAAGGGCGAGGAG | 274 |
CTTCGGGCATGGCGGACTTGA | ||
GFP 3′ | TATCATGGCCGACAAGCAGAAGAACG | 230 |
CGGCGGCGGTCACGAACTCC | ||
GFP end-point | GTCCAGGAGCGCACCATCTTCTT | 406 |
CGGCGGCGGTCACGAACT | ||
Nanog | TCCAGAAGAGGGCGTCAGAT | 283 |
CTTTGGTCCCAGCATTCAGG | ||
β-actin | TTGTTACCAACTGGGACGACA | 350 |
TCTTCATGAGGTAGTCTGTCA | ||
Gata-4 | AGGCGAGATGGGACGGGACACTAC | 202 |
CGCAGGCATTACATACAGGCTCAC | ||
Wnt-3 | CTTCATGATCGCCGGCAAACTTC | 189 |
TGGGATGGAGCCGCAGAGCAGAG | ||
Jag-1 | CAGTGCCTCTGTGAGACCAAC | 193 |
AGGGGTCAGAGAGACAAGCATG | ||
Jag-2 | GCAAAGAATGCAAAGAAGCC | 233 |
TGGCTGCCACAGTAGTTCAGG |