Table 3.
Culture-dependent and independent viability indicators
| 2log ratios μ-array | 2log ratios q-PCR | ||||||||||
| T(°C) | CFU(10log) | LT(h) | PM (%) | 16S/23S | dnaK | gspA | cotJA | dnaK | gspA | cotJA | 23S rRNA |
| 30 | 8.3 | 0 | 0 | 0.9 | 0.1 | - | -0.2 | 0.0 | 0.0 | 0.0 | 0.0 |
| 40 | 8.4 | 0.2 | 1 | 0.9 | 0.5 | 0.9 | 0.0 | 0.2 | 3.9 | -0.3 | -0.3 |
| 50 | 8.4 | 0.8 | 1 | 0.7 | 2.0 | 6.1 | 0.4 | 1.1 | 5.5 | 1.2 | 0.0 |
| 57 | 8.2 | 4 | 2 | 0.4 | 2.4 | 4.3 | 1.2 | 0.0 | 4.8 | 1.1 | -0.1 |
| 58 | 7.7 | 8 | 3 | 0.3 | 1.6 | 1.8 | 2.9 | -1.0 | 3.7 | 1.8 | 0.0 |
| 59 | 5.1 | 9 | 5 | 0.2 | 0.6 | 0.8 | 3.8 | -1.9 | 3.8 | 2.7 | 0.2 |
| 60 | 4.3 | 9 | 6 | 0.2 | -1.0 | -0.4 | 4.5 | -2.9 | 0.0 | 2.5 | 0.4 |
| 62 | 2.5 | 12 | 25 | 0.1 | -2.0 | -1.2 | 4.8 | -4.1 | 0.6 | 3.3 | 0.4 |
Listed in columns is the applied temperature, the number of colony forming units (CFU) in 10log units, the lag time (LT) of the growth curves in hours (h), the number of cells with a propidium iodide permeable membrane (PM) expressed in percentage (%), the ratio between 16S and 23S ribosomal rRNA as determined by gel image analysis, the 2log ratios of expression levels at the indicated temperature in the first column and the reference temperature at 30°C for dnaK, gspA and cotJA genes (determined by microarrays), and the 2log ratios of expression levels at the indicated temperature in the first column and the reference temperature at 30°C of transcripts for dnaK, gspA, cotJA and 23S rRNA genes (determined by quantitative PCR with primer probe combinations listed in Table 2).