Table 1 Primer information for PCR amplification and sequencing of WFS1.

Exon		Forward primers		Reverse primers		Size (bp
1		CGGAGATGTGGAGTGATTGG		GACCTTGTCGGGACTTTGG		432
2		GCATGTGCAGGTCTGAGATT		ACAAAGGTGACAGGTGCAAG		679
3		CACCTCACCCGCATAGAGTT		GCCCAAGATGAGGTGTGAGT		571
4		CACCTTCCTCACCGTGTTTT		ACCACACAAGCCAAAGAGAA		578
5		CGTCTGGTGGGAGACCAGT		GGTCTCTACAGGAAGGTTCTGG		576
6		CTGAGTCCACCCCAGCTACT		GGTCTACTCCCAGCGTCCA		500
7		CCTGGTCCTCAACCCTCAG		CTCCCGAGGACACATCCTTA		499
8:1		AGGGTAGGACCACTAGGATGG		CTTCAGGTAGGGGCAATTCA		735
8:2		TATGCCCATTTCCTGCTCTC		TACTGCTGCCAGGTCAGTGT		794
8:3		GTCTGTAGTGTGCCCCTGCT		GATGGTGCTGAACTCGATGA		675
8:4		TTCGACCGCTACAAGTTTGA		TGTGGAGGTTACACAGATGCTT		783
Primer information for PCR amplification and sequencing of GJB2
1		GCACTTGGGGAACTCATGG		GCAACCGCTCTGGGTCTC		466
2		TCAGAGAAGTCTCCCTGTTCTGTCC		TGAGCACGGGTTGCCTCATC		833
		CTGGCTCACCGTCCTCTTCATTTATGGGCCCTGCAGCTGATC		CGCATCGAAGGCTCCCTGT		†
Primer information for PCR amplification and sequencing of GJB6 exon 3
3:1		AGACTAGCAGGGCAGGGAGT		CCCCTCTATCCGAACCTTCT		526
3:2		TGCATGTGGCCTACTACAGG		GGTTGGTATTGCCTTCTGGA		577
Primer information for PCR amplification of del(GJB6‐D131830) large deletion*
Deletion		TTTAGGGCATGATTGGGGTGATTT		CACCATGCGTAGCCTTAACCATTTT		460
Normal				AGCCTTTATGTATGTGTTTTACTTCCTT		267
Primer information for PCR amplification and sequencing of OPA3
1		AGGATGCGCCTCTGAAGTT		CCACTATTGGCCACTGGACT		415
2		TACCCAGGCTAGGTGACTGC		GCATCAAGATCCTGGTGGTT		604

*Primers identical to Del Castillo et al 2002²⁵ ; †Internal sequencing primers.