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. 2009 Jan 5;18(6):1006–1016. doi: 10.1093/hmg/ddn428

Figure 6.

Figure 6.

Reduced PGC-1α expression and activity adipocytes expressing mutant huntingtin. (A) mRNA levels for PGC-1α and PGC-1β were determined by qRT–PCR in adipose tissue from R6/2 Tg mice at the ages indicated for five mice of each genotype. Expression levels were normalized to HPRT and TBP. (B) PCG-1α co-activator activity for PPARγ was assessed in the presence of Htt-Q25 and Htt-Q103. 3T3-L1 pre-adipocytes were co-transfected with a luciferase reporter construct containing the multimerized (3×) PPAR-responsive element upstream of the viral thymidine kinase minimal promoter in the indicated combinations with PPARγ/RXRα, PGC-1α and Htt-Q25 or Htt-Q103 huntingtin expression vectors. All samples also received a Renilla luciferase expression vector as a normalization control. The luciferase activity produced from the PPAR-responsive reporter gene are shown as the average ± SD of four replicates from a representative experiment. *P < 0.05; **P < 0.01 for comparisons indicated.