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Effect of actinomycin D treatment on SP distribution. Confocal analysis of HeLa cells, 24 hours post-transfection, treated (or not) with 5 μg/ml Actinomycin D (ActD/no ActD) for 2 hours. Prior and during the experiment, cells were incubated with CHX at 100 μg/ml. Cells expressing SP75-RFP (A), SP94-RFP (B) or GFPcORF (C) were fixed and co-immunostained for B23/nucleophosmin nucleoli marker. White bar = 10 μm.
