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ET2+3 – hIRP1 binding interaction concentration dependence. (A) EMSA showing the hIRP1 concentration dependence on binding to ET2+3. Lanes showing hIRP1 concentrations of 74 pM-5.1 nM were omitted for clarity. (B) Plotted data used to determine the Kd for the IRP1-ET2+3 interaction.
