Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Mar 6;284(10):6348–6360. doi: 10.1074/jbc.M807040200

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2009, The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 7. — 8-Br-cGMP inhibits U-46619-induced MYPT1 association with the detergent-soluble membrane fraction. A, representative Western blots of the subcellular distribution of MYPT1 following U-46619 stimulation with and without 8-Br-cGMP pretreatment. Membranes reprobed for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and vimentin indicate minimal contamination of the detergent-soluble (Det. Soluble) fraction from either the cytosolic or pellet (detergent-insoluble) fractions. B, quantification of changes in MYPT1 subcellular distribution indicates U-46619 stimulation significantly increases MYPT1 association with the detergent-soluble fraction. Pretreatment with 8-Br-cGMP completely inhibits the U-46619-induced association of MYPT1 with the detergent-soluble fraction. Rabbit cerebral vessels were used for the fractionation assay because of protein requirements. Error bars represent mean ± S.D.; ^*, p < 0.05 with respect to control, n = 5.