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Bacterially expressed Swr1(1–681) binds to H2AZ-H2B in vitro. A, binding of recombinant H2AZ-H2B or H2A-H2B dimers to immobilized, His6-tagged Swr1(1–681) was assayed by Western blotting using anti-H2B. B, binding of recombinant H2AZ-H2B dimers to immobilized, bacterially expressed Swr1(1–681), Swr1(371–678), and Swc7 was determined by Western blotting using anti-H2B.
