FIGURE 1.

Running start assays. A preincubated solution of Dpo4 (100 nm) and 5′-³²P-labeled DNA (100 nm) was rapidly mixed with all four dNTPs (200 μm each), and the reaction was quenched with 0.37 m EDTA at various time intervals. A, 17/26-mer. B, 17/26-mer-dG^AP. Sizes of important products are indicated, and the 21st position marks the location of the dG^AP lesion from the 3′-terminus of the DNA template.