TABLE 2.

Binding affinity of Dpo4 to damaged and control DNA substrates at 23 °C

DNA substrate	Damaged DNAa	Control DNAb	Affinity ratioc
	nm	nm
19/26-mer	2.7 ± 0.2	3.1 ± 0.5	1.2
20/26-mer	1.0 ± 0.1	4.0 ± 0.2	4.0
21/26-mer	4.4 ± 0.2	3.7 ± 0.2	0.8
22/26-mer	2.6 ± 0.2	3.8 ± 0.6	1.5
23/26-mer	4.0 ± 0.3	3.6 ± 0.5	0.9

^aDamaged DNA refers to those with template 26-mer-dG^AP in Table 1

^bControl DNA refers to those with template 26-mer in Table 1

^cValues were calculated as (K_{d, DNA})_control/(K_{d, DNA})_damaged