TABLE 3.

Kinetic parameters for single dNTP incorporation opposite template 26-mer-dG^AP

dNTP	Kd, dNTP	kp	(kp/Kd, dNTP)damaged	Efficiency ratioa,b	Fidelityc	Probabilityd
	μm	s–1	μm–1 s–1
19/26-mer-dGAP
dGTP	187 ± 36	4.3 ± 0.3	2.3 × 10–2	1.1		99.8
dATP	373 ± 81	(6.3 ± 0.5) × 10–3	1.7 × 10–5	0.8	7.4 × 10–5	0.1
dCTP	227 ± 23	(6.4 ± 0.2) × 10–3	2.8 × 10–5	5	1.2 × 10–3	0.1
dTTP	1180 ± 190	(1.0 ± 0.1) × 10–2	8.7 × 10–6	1.0	3.8 × 10–4	0.0
20/26-mer-dGAPe
dCTP	167 ± 15	1.03 ± 0.03	6.2 × 10–3	9.2		98.4
dATP	856 ± 184	(7.6 ± 0.8) × 10–2	8.9 × 10–5	0.2	1.4 × 10–2	1.4
dGTP	955 ± 160	(2.0 ± 0.2) × 10–3	2.1 × 10–6	21	3.3 × 10–4	0.0
dTTP	557 ± 36	(5.5 ± 0.1) × 10–3	9.9 × 10–6	8.4	1.6 × 10–3	0.2
21/26-mer-dGAPe
dGTP	674 ± 231	(2.8 ± 0.3) × 10–2	4.2 × 10–5	88		88.6
dATP	886 ± 145	(2.7 ± 0.2) × 10–3	3.1 × 10–6	0.8	6.8 × 10–2	6.5
dCTP	328 ± 79	(6.8 ± 0.5) × 10–4	2.1 × 10–6	15	4.7 × 10–2	4.4
dTTP	2300 ± 461	(4.9 ± 0.7) × 10–4	2.2 × 10–7	8.2	5.1 × 10–3	0.5
22/26-mer-dGAP
dCTP	682 ± 80	6.3 ± 0.3	9.3 × 10–3	3.7		99.8
dATP	826 ± 85	(4.2 ± 0.2) × 10–3	5.0 × 10–6	0.8	5.4 × 10–4	0.1
dGTP	502 ± 98	(4.3 ± 0.3) × 10–3	8.6 × 10–6	0.8	9.3 × 10–4	0.1
dTTP	1540 ± 257	(4.7 ± 0.5) × 10–3	3.1 × 10–6	3.6	3.3 × 10–4	0.0
23/26-mer-dGAP
dGTP	62 ± 18	1.9 ± 0.1	3.1 × 10–2	0.8		98.9
dATP	668 ± 173	(3.7 ± 0.4) × 10–2	5.5 × 10–5	0.3	1.8 × 10–3	0.2
dCTP	1130 ± 161	(9.0 ± 0.7) × 10–3	7.9 × 10–6	0.2	2.6 × 10–4	0.0
dTTP	1290 ± 218	(3.4 ± 0.3) × 10–2	2.7 × 10–4	0.01	8.7 × 10–4	0.9

^aCalculated as (k_p/K_{d, dNTP})_control/(k_p/K_{d, dNTP})_damaged

^bValues for (k_p/K_{d, dNTP})_control are listed in supplemental Table 1

^cCalculated as (k_p/K_{d, incorrect dNTP})_damaged/(k_p/K_{d, correct dNTP})_damaged + (k_p/ K_{d, incorrect dNTP})_damaged

^dCalculated as ((k_p/K_{d, dNTP})_damaged/(Σ(k_p/K_{d, dNTP})_damaged)) × 100

^eDenotes pause sites