. 2009 Mar 6;284(10):6379–6388. doi: 10.1074/jbc.M808161200

TABLE 4.

Biphasic kinetic parameters for correct dNTP incorporation into 5′-³²P-labeled DNA (30 nm) in the presence of a DNA trap (5 μm) at 37 °C

DNA substrate	Correct dNTP	A₁	k₁	A₂	k₂
		nm	s^–1	nm	s^–1
19/26-mer	dGTP	27 ± 1 (91%)^a	3.2 ± 0.4
20/26-mer	dCTP	26 ± 1 (86%)^a	4.6 ± 0.5
21/26-mer	dGTP	27.0 ± 0.4 (86%)^a	2.3 ± 0.1
19/26-mer-dG^AP	dGTP	27.0 ± 0.4 (89%)^a	2.5 ± 0.2
20/26-mer-dG^AP	dCTP	2.0 ± 0.7 (6.7%)^a	11 ± 6	18 ± 1 (60%)^a	0.7 ± 0.1
21/26-mer-dG^AP	dGTP	0.9 ± 0.1 (3%)^a	1.9 ± 0.3	5.7 ± 0.2 (19%)^a	0.031 ± 0.003

Calculated as (reaction amplitude/30 nm) × 100