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Region of mouse Retn between –8872 and –8676 functions as an adipocyte-specific enhancer. A, luciferase activity of Retn reporter constructs transfected into 3T3-L1 adipocytes. B, luciferase activity from 3T3-L1 adipocytes, HEK 293T, and HepG2 cells transfected with a plasmid whereby the mouse Retn enhancer (–8872 to –8676 bp) drives reporter gene expression. Data presented here and in all subsequent transfections are expressed as mean ± S.E. (n = 3 per condition).
