FIGURE 4.

The Retn enhancer contains three C/EBP-binding sites and one PPARγ-binding site. A, sequences with similarity to C/EBP- and PPARγ-binding motifs are underlined in the mouse Retn enhancer region. Comparison of the putative binding sites to the consensus C/EBP and PPARγ motifs is shown. B, schematic representation of the oligonucleotide probes used for this study, with putative C/EBP- and PPARγ-binding sites indicated. C–E, EMSA of activities within 3T3-L1 preadipocyte (Pread) or adipocyte (Ad) nuclear extract (NE) and the indicated probes. F, EMSA with in vitro translated PPARγ/RXRα. For the indicated reactions, antibody (Ab) was added to the nuclear extract or in vitro translated protein ∼10 min before the addition of probe DNA.