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. 2009 Mar 6;284(10):6116–6125. doi: 10.1074/jbc.M808407200

FIGURE 7.

FIGURE 7.

Human RETN lacks a functional PPARγ-binding site. A, sequence alignment of the mouse, rat, and human resistin genes based on comparative genomic analysis. Sequence coordinates are relative to the resistin TSS for each species. C/EBP and PPARγ motifs from the mouse adipocyte enhancer are underlined. B, EMSA examining binding of in vitro translated PPARγ/RXRα to the mouse PPARγ-binding site (mouse) or the corresponding region of human RETN (human). C, luciferase activity of transfected plasmids in 293T cells. The human sequence corresponding to the mouse enhancer (–13262 to –13110 bp, hRETN-Enh) is fused to a luciferase reporter vector and cotransfected with or without C/EBPα and PPARγ/RXRα expression vectors. D, luciferase activity of transfected plasmids in 3T3-L1 adipocytes. Reporter gene expression is driven by the mouse enhancer (–8843 to –8695 bp, mRetn-EnhC) or the corresponding sequence of human RETN (hRETN-Enh).