FIGURE 1.

Sequence alignments and NMR data for Na_V1.2 and Na_V1.5 CTDs. A, sequence alignment of Na_V1.2 (1777-1882) and Na_V1.5 (1773-1879) CTDs, with 83% identity and 93% similarity. Non-conservative substitutions are shown in bold type. B, medium range ¹H-¹H NOEs. C, secondary structure elements predicted from chemical shifts using TALOS (49) are shown as bars for α-helices and arrows for β-strands. {¹H}-¹⁵N steady-state NOE (D) and secondary ¹³C^α chemical shifts for Na_V1.2 CTD (E) indicate a well folded domain encompassing residues Leu-1790—Glu-1868. F, ¹H,¹⁵N HSQC (right panel) with expansion of the central region (left panel) of Na_V1.2 (1777-1882). The W1802^ε1 resonance is aliased in the ¹⁵N dimension from 131.5 ppm.