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. 2009 Mar 6;284(10):6520–6529. doi: 10.1074/jbc.M807564200

FIGURE 2.

FIGURE 2.

Loss of prx1 expression is associated with a compensatory increase in Prx5. A, total cell lysates from prx1+/+ and prx1-/- MEFs were immunoblotted and probed with antibodies against the indicated Prx family members or against β-tubulin as a loading control. B, quantitative real time PCR analyses of transcript levels for individual members of the peroxiredoxin family. Samples were run in triplicate and expressed as a mean relative expression ratio when compared with glyceraldehyde-3-phosphate dehydrogenase ± S.E. *, p value was significant (p = 0.012). C, Prx5 immunoblots of prx1-/- MEFs stably transduced with a lentiviral-prx1 vector or control vector. Note that the reexpression of Prx1 was associated with a concurrent reduction/normalization of Prx5. D, immunostaining for prx5 in prx1+/+ and prx1-/- MEFs. A FITC-tagged secondary antibody was used for visualization. The bottom panel shows the same cells counterstained with 4′,6-diamidino-2-phenylindole to emphasize the nucleus. Note the stronger signal in prx1-/- MEFs, which is mostly diffuse cytoplasmic staining. Some cells display punctate cytoplasmic staining, which is shown by the arrow in the inset of the top left panel.