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The cellular redox state does not influence Prx5 expression. A, prx1-/- cells were grown under standard conditions and then incubated with increasing amounts of the antioxidant N-acetylcysteine for 24 h. Prx5 immunoblots were then performed. B, the same cells were incubated for 1 or 3 h in the indicated concentrations of H2O2 and then immunoblotted for Prx5. β-Tubulin was used as a loading control in both A and B.
