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. 2009 Mar 6;284(10):6520–6529. doi: 10.1074/jbc.M807564200

FIGURE 6.

FIGURE 6.

Differential prx5 promoter occupancy by c-Myc and Prx1 in prx1+/+ and prx1-/- MEFs. A, diagrammatic representation of the murine prx5 gene promoter showing the location of two perfect consensus E-box elements (CACGTG) (-1047 (site D) and -310 (site F) relative to the start of transcription) and two alternate elements CATGTG (-1319 (site C) and -606 (site E)) oriented in the opposite direction of the D and F sites. The arrows indicate the approximate positions of primers used to amplify each site (see supplemental Table 2 for the sequences of all primers). Sites A and B are non-E-box-containing sites that were used in control quantitative PCR amplifications. B, ChIP of the prx5 promoter in both prx1+/+ and prx1-/- cells was performed with anti-c-Myc antibodies to identify sites of c-Myc occupancy. *, p value was significant (p < 0.05) when comparing signals from prx1-/- cells and prx1+/+ cells. The horizontal line represents an arbitrary value below which the signal is believed to represent background. C, ChIP of the prx5 promoter was performed and displayed as described for B except that an anti-Prx1 antibody was used to identify sites of Prx1 occupancy.