FIGURE 4.

A, relative cDNA copy numbers of CypA, CypB, and CypC obtained from the quantitative RT-PCR of RNA extracted from clone C cells as described under “Experimental Procedures.” B, immunoblot of protein lysates from rabbit kidney medulla and equal amounts of protein (36 μg) from clone C cell lysate and conditioned medium of high density cells with anti-CypA (left), anti-Cyp B (middle), and anti-Cyp C (right) antibodies. C, immunoblot of equal amounts of protein (41, 38, and 39 μg) from the conditioned medium of three independent Transwell filters probed with anti-CypA and anti-CypC antibodies.