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. 2009 Mar 6;284(10):6038–6042. doi: 10.1074/jbc.C800207200

FIGURE 1.

FIGURE 1.

CXCL8/IL8 induces the up-regulation of VEGF in endothelial cells through CXCR2. A, serum-starved SVEC cells were treated for the indicated times with 100 ng/ml CXCL8/IL8 or exposed to 1% O2. Vegfa mRNA levels were determined by quantitative PCR, and cellular (B) and secreted (C) VEGF levels were determined in lysates and culture media, respectively. pVEGFR2, phosphorylated VEGFR2. D, serum-starved SVEC cells were pretreated (30 min) with vehicle (Control) or 50 nm SB-225002 (CXCR2inh), stimulated for 12 h with phosphate-buffered saline (Control) or 100 ng/ml CXCL8/IL8, and analyzed by Western blot (WB). E, SVEC cells were infected with lentiviruses encoding for GFP and murine CXCR2 shRNA or control shRNA. Cells were selected by fluorescence-activated cell sorter. Two groups showed diminished CXCR2 levels (shRNA1 and shRNA3) by Western blot analysis, whereas another displayed inefficient knockdown (shRNA2) and served as an additional control. F, analysis of VEGF up-regulation in response to 12 h of treatment with 100 ng/ml CXCL8/IL8 in SVECs with different CXCR2 expression. ANOVA test, *, p < 0.01, **, p < 0.001.