FIGURE 4.

Requirement of the CBM complex for CXCL8/IL8-induced up-regulation of VEGF. A, siRNA-mediated knockdown of Carma3, Bcl10, Malt1, and Tak1 in SVECs, as judged by Western blot (WB) analysis for each protein, using tubulin as a loading control. B and C, luciferase assays of cells transfected with the indicated siRNAs and NFκB luciferase and Renilla luciferase encoding plasmids at day 4. Cells were serum-starved and stimulated with CXCL8/IL8 (100 ng/ml) or TNFα (10 ng/ml) for 12 h as indicated. D and E, their Vegfa mRNA (D) and secreted VEGF levels (E) are represented. F, Western blots for VEGF, phospho-VEGFR2 (pVEGFR2) and total VEGFR2 after 12 h treatment with 100 ng/ml CXCL8/IL8 in cells transfected with siRNAs. ANOVA test, *, p < 0.01.