FIGURE 5.

MILI interacts with eIF3a. A, purified anti-MILI peptide antibody (Ab) efficiently immunoprecipitates (bound) MILI from a testicular extract. Adding the antigen-peptide blocks the precipitation. B, eIF3a is specifically co-precipitated by with MILI, as revealed by mass spectrometry. C, Western blot showing that the MILI antibody co-immunoprecipitated MILI and eIF3a from a testicular extract. Adding the blocking peptide almost completely prevented the immunoprecipitation of both MILI and eIF3a. However, adding RNase A did not disrupt the co-precipitation. D, Western blot showing that the eIF3a antibody, but not actin or T7 antibodies, specifically co-precipitated eIF3a and MILI from a testicular extract. Adding RNase A did not disrupt the co-precipitation.