FIGURE 7.

Effect of CKII inhibitors on cytoadherence. The effect of TBB (a), DMAT (b), and TBCA (c) on binding of FCR3-CSA to CSA (CSA panels) and ITG-ICAM to ICAM-1 (ICAM-1 panels). Parasitized RBCs were treated for 30 min with a 100 μm concentration of each of the CKII inhibitors, as indicated. iRBC suspensions (1% hematocrit and 3% parasitemia) were flowed over receptor-coated microslides for 5 min. The flow rate was set to 0.18 ml/min. The number of stationary or rolling iRBCs was counted in six random fields on the microslides from three independent experiments. Results were expressed as percentage bindingin comparison with untreated culture (100% binding). Microslides coated with PBS were used as negative controls in these assays. Granulated gray bars represent data from static assays, whereas solid gray bars represent data from flow assays.